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Microsoft Office 2013 Crack. November 30, 2016 . Office 2013 Crack is an important Microsoft Office product that allows all users to work on documents, spreadsheets, and presentations through a .
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Onix is a Raman spectroscopy device that is used for predictive maintenance of underground coal mining equipment. The system can collect data, and then process the data to provide a quantitative measure of the wear of underground mining equipment. The data can be examined to identify early warning signs of equipment failure. When the equipment reaches the point of failure, the data can be used to assess the amount of remaining useful life.-RT system. PCR positive reactions were detected using the Big Dye kit in a 3500 Dx Genetic Analyzer (Perkin-Elmer, Norwalk, CT). The results were analyzed using Sequencing Analysis v5.2 software (Applied Biosystems, Foster City, CA) and verified by visual inspection.
Interphase FISH
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FISH was performed as previously described \[[@B34]\]. FISH experiments were performed on metaphase spreads and interphase nuclei from wt and *p53*^*-/-*^cells. Probes were made from a PCR clone for the ***BRAC1***gene (RP11-4P17) in pBeloBACter-1 (Genome Systems, St Louis, MO) using BAC ends (Invitrogen, Carlsbad, CA). BAC DNA was isolated from the BAC-CEP 17 1.2Mb contig using the NucleoSpin DNA/RNA isolation kit (Macherey-Nagel, Bethlehem, PA). For *BRAC1*FISH, 5 μg of pBeloBACter-1 DNA (0.5 μg/μl) was labeled using a nick translation kit (Dyomics, Jena, Germany) according to the manufacturer’s instructions. A digoxigenin-labeled riboprobe was synthesized using the DIG nick translation kit (Sigma, St Louis, MO) according to the manufacturer’s instructions. FISH was performed on metaphase spreads
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