BD Reauthor Pro 3.3.2 (3D)
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MicroRNA-200a (miR-200a) has been reported to participate in cancer development by modulating target genes. However, its role in human glioma remains unknown. The aim of the present study was to investigate the potential of miR-200a in the control of human glioma invasion and migration. Effects of miR-200a on the migration and invasion of U87 and U251 cells were examined by wound healing assay and transwell chamber assay. The protein level of matrix metalloproteinase 9 (MMP9) was detected by western blot. The regulation of MMP9 expression in U251 cells was investigated by transfection of MMP9 small interfering (si)RNA. The expression of miR-200a and MMP9 in U251 cells transfected with miR-200a agomir was determined by real-time polymerase chain reaction (RT-PCR). The expression of miR-200a and MMP9 in U
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