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A simplified method for the detection of immunoglobulins by Quantitative Disc Electrophoresis (QD-EP) is described. This method is only applied to the initial phase of the preparation of the sample where the immunoglobulins of interest are found in appreciable quantities, the electrophoretic separation is as short as necessary, and the identification of the subsequent separation from the initial run is facilitated. The method requires only two capillary tubes: the one containing the sample is separated from the one containing the immunoglobulin standards. Identification of immunoglobulins is carried out by a densitometry technique. A computer can be used for the data elaboration. A series of 99 serum samples and a series of 80 toliar standard sera were tested by this method and results were good with the 99 sera and with the sera of the toliar standard series. An example of immunodeficiency in a mentally retarded boy is shown.Spirulina is a blue-green microalgae that is different from other microalgae: it grows at high temperatures and high salinities, and it contains high concentrations of proteins, enzymes, vitamins, minerals, and anti-oxidants. Spirulina has recently gained attention as a feed ingredient due to its demonstrated ability to improve growth, improve immune status, and improve stability of animal products. Spirulina-enriched feed has been used in various animal species, including poultry, swine, aquaculture, ruminants, and egg-layer hens. The applications of Spirulina as a feed additive show promising results, and it is used as an additive in feed products. In most cases, however, the supplementation of Spirulina is done in conjunction with another microalgae-based product. For example, Spirulina is used together with Chlorella or other microalgae, in order to increase the level of Spirulina in the feed.
The ability of Spirulina to improve animal nutrition and grow fast has led to the analysis of its composition. In addition, the ability of Spirulina to improve animal immune systems has provided researchers with
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