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RNA interference (RNAi) is an extremely powerful and versatile method to inhibit target mRNA and protein synthesis. Previous studies showed that RNAi could also be used to specifically knock down the expression of different growth factor receptors as this did not directly interfere with their function. We demonstrate that RNAi technology can be used to study growth factor receptor function in cells. In particular, we successfully knocked down the expression of the epidermal growth factor receptor (EGFR) in different cell lines using a short hairpin RNA (shRNA) strategy. Reduction of EGFR expression levels in HeLa cells by RNAi resulted in a significantly diminished cell growth and a dramatically decreased clonogenicity. The importance of the EGFR kinase activity in the observed phenotypes was assessed by using specific kinase inhibitors. The down-regulation of EGFR also resulted in a reduced invasion potential, indicating that the invasion process is dependent on the EGFR signaling. In summary, we present here a gene silencing strategy that might be very useful for studies on the function of different growth factor receptors.The actor and comedian known by millions of people as “the geek” has been named the official 2020 Super Bowl Host.
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Stephen Colbert is one of the four host candidates.
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In addition to Colbert and the four host candidates, the NFL will announce the Super Bowl LIV Halftime Show performers on Friday.
Super Bowl LIV will be held Feb. 2 at Hard Rock Stadium in Miami Gardens, Florida, with a kickoff time of 3:30 p.m. ET. ESPN will broadcast the game.
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