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Glioma-derived neurospheres represent an appropriate model for mechanistic drug evaluation on CNS stem/progenitor cells in vitro, as they represent the self-renewing cell population that sustains the malignant phenotype of glioma cells. However, their use as in vitro models has been limited to date by the fact that traditional neurosphere protocols require relatively high cell plating numbers that may be cytotoxic, and/or by limitations in batch-to-batch reproducibility. To address this issue, a protocol was developed that uses a relatively low-cell-density plating protocol, which previously has not been reported for the glioma-derived neurosphere model. This study describes the first assessment of the cytotoxic effects of cell plating and culture techniques on glioma-derived neurospheres. Cytotoxicity was assessed in terms of fold-change in viable cell number during the course of culture and by morphological analysis of neurospheres plated at different densities. These experiments demonstrate that neurospheres can be grown for multiple passages under conditions that minimize the potential for cytotoxicity. These findings provide a solid foundation for the further development of this model system, and they have implications for the wider use of this model system for drug discovery in CNS stem/progenitor cell-based assays.This is just a quick (very quick) post-CPP demonstration of a simple RIA (Silverlight 4) which shows how to create a UserControl that inherits from UserControl:
After this post, we have our UserControl:
UserControl Code Behind:
Back to the XAML:
Back to the Page in which we have all this stuff:
And we can also use this UserControl in a another Page by setting it’s template (which will be inherited by the other UserControl):
And finally, let’s create a simple animation:
Conclusion
I hope this post is a good start to your learning in Silverlight.
Kudos
Find the source code for this post here:
Watch for updates in the user control.
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How to Fix “Failed to create windows socket as user “NT AUTHORITY/SYSTEM”?
I am running a node app on my Windows 7 home computer and keep getting the error:
Failed to create windows socket as user “NT AUTHORITY/SYSTEM”. (Error
code: 80070005)
With subsequent crashes of the node app.
I have read many of the threads here on SO but have not solved the problem.
From
sockets/stream.js:245
throw new Error(‘Failed to create windows socket as user “‘ +
nss
[“NT AUTHORITY/SYSTEM”] + ‘”. (Error code:’+ e.code + ‘)’);
^^^^^^^^^^^^
Error: Failed to create windows socket as user “NT AUTHORITY/SYSTEM”. (Error code: 80070005)
at Object.module.exports.createSocket (sockets/stream.js:245:14)
at Socket.connect (c:\projects\myproject\